One day hands on workshop was organized by Department of Biotechnology for undergraduate students entitled “DNA isolation, PCR amplification and Stem cell in Cancer Research” on 4th April 2017. This workshop was conducted by Helix Biogenesis Pvt. Ltd. The purpose of the workshop was to bring hand on experience on molecular techniques like PCR, agarose gel electrophoresis and Genomic DNA isolation, Stem cells and their application in diagnostic and therapeutic. A galaxy of eminent researchers which included Dr. Ravindra Kumar ( Assistant Prof. Department of Botnay, Hindu College, Delhi) and Dr. Rashmi Bharadwaj ( Assistant Prof. MDM University Rohtak) and Dr. Amit (Assistant Prof. Delhi University) added sparkles to the forum.
The initial session was started with an interactive session in which Dr. Rashmi Bharadwaj shared the basic knowledge about stem cells, their types, source, their derivation and use of embryonic stem cells lines. She also included the culturing of stem cells obtained from bone marrow, umbilical cord, etc, their storage and their identification by organ specific, surface markers known as CD markers ( clusters of differentiation)
She also delivered the valuable insights on the ongoing research based on stem cells for treatment of many diseases like cancer, neurological disorders, HIV, AIDS, Spinal cord injury, liver diseases, corneal diseases and more interestingly baldness.
She also gave information about Prokerna contact lens which are simple contact lens loaded with stem cells that could migrate and transplant in the eyes and restore and repair the human sight. She also highlighted the iconic accomplishment of two noble prize winning scientists Sir John B. Gurdon and Shinya Yamanaka for turning human skin cell to stem cell. At the end of this session she also focused on challenges being faced in embryonic stem cell research and solved many queries of students and faculty members based on stem cell and their application in medical sciences.
The second session was conducted in lab where undergraduate students got hand on exposure on molecular techniques basically genomic DNA isolation from bacterial culture followed by analysis of extracted genomic DNA by agarose gel electrophoresis. In the other part of this session they performed DNA amplification by Polymerase Chain Reaction followed by electrophoresis to check the amplification. All the technical aspects, principles behind every steps and loop holes were well explained by the experts from Helix BioGenesis. This workshop has helped the students to develop clear insight on molecular techniques.